Significant changes to the article as accepted for publication will only be considered at this stage with permission from the Editor. Langbein L., Rogers M.A., Praetzel-Wunder S., Bckler D., Schirmacher P., Schweizer J. The authors gracefully acknowledge Ayesha aslam for providing curly hairs and Sana batool for providing hairs used in testing the mechanical properties of hair. Recheck references in the text against reference list after your manuscript has been revised. Video and animation files supplied will be published online in the electronic version of your article in Elsevier Web products, including ScienceDirect. At manuscript submission, authors must provide the CONSORT checklist accompanied by a flow diagram that illustrates the progress of patients through the trial, including recruitment, enrollment, randomization, withdrawal and completion, and a detailed description of the randomization procedure. The ability of K31 to straighten the curly hair and repairing the chemically damaged hair was investigated. This information must also be inserted into your manuscript under the acknowledgements section with the headings below. In order to allow us to create links to abstracting and indexing services, such as Scopus, Crossref and PubMed, please ensure that data provided in the references are correct. Acknowledgements Collate acknowledgements in a separate section at the end of the article before the references and do not, therefore, include them on the title page, as a footnote to the title or otherwise. Content should make no assumptions about the beliefs or commitments of any reader; contain nothing which might imply that one individual is superior to another on the grounds of age, gender, race, ethnicity, culture, sexual orientation, disability or health condition; and use inclusive language throughout. Hair care products: waving, straightening, conditioning, and coloring. 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JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Registration of clinical trials Registration in a public trials registry is a condition for publication of clinical trials in this journal in accordance with International Committee of Medical Journal Editors recommendations. The alkali treated hair on K31 treatment efficiently straightened the curly hair (Fig. The text should be in single-column format. Trials must register at or before the onset of patient enrolment. Previously a preparation of keratin peptides of thirteen amino acids in an organic solvent was reported to straighten the curly hair, with improved diameter and tensile strength [25]. However, if specifically requested by the author(s), plates may be reproduced larger than the typeset area; all originals for these should have the same proportions to achieve uniformity in their presentation. Health improvement of human hair and their reshaping using recombinant If you decide to participate, a new browser tab will open so you can complete the survey after you have completed your visit to this website. 4). Corresponding authors who have published their article open access do not receive a Share Link as their final published version of the article is available open access on ScienceDirect and can be shared through the article DOI link. Langbein L., Yoshida H., Praetzel-Wunder S., Parry D.A., Schweizer J. They should be no longer than 4000 words and 40 references (as above, please note that word count also excludes tables, figures and legends). For more information, please review the Elsevier Policy on the Use of Images or Personal Information of Patients or other Individuals. Application of K31 resulted in marked improvements in smoothness, diameter and mechanical strength of the damaged hair. Written consents must be retained by the author but copies should not be provided to the journal. For more detailed instructions please visit our video instruction pages. Hot topics: These tend to be invited papers but unsolicited Hot topics are welcome. If these references are included in the reference list they should follow the standard reference style of the journal and should include a substitution of the publication date with either 'Unpublished results' or 'Personal communication'. Five fibers from each of the hair tress were randomly selected and incubated in 15ml 0.05M Tris-Cl buffer (pH 7.5) containing 0.2mgml1 K31protein in a petri plate for 1h in an orbital shaker at 120rpm. Please have a look at the examples here: example Highlights. consistently. We recommend avoiding the use of descriptors that refer to personal attributes such as age, gender, race, ethnicity, culture, sexual orientation, disability or health condition unless they are relevant and valid. Journal of Global Antimicrobial Resistance - ScienceDirect The alkali was washed out and the hairs were extended into straight configuration with the help of a comb. Keratin K31 when analyzed by CD spectrum at 37C showed a positive peak at 193nm and 2 negative peaks at 212nm and 223nm (Fig. Hair, being one of the most important components of the beauty care processes, attracts the use of a variety of hair treating cosmetics. Previous studies have reported that cortex of the hair are mainly responsible for the mechanical strength of the hair and this strength is weakened by bleaching [36,37]. The hair sample after treating with 10% bleach solution [24] for 1h in shaking incubator was rinsed with water and then air dried. We produced recombinant Keratin K31, refolded and purified for studying its role in reshaping hair and treatment of damaged hair. Highlights Highlights are mandatory for this journal as they help increase the discoverability of your article via search engines. Article transfer service This journal uses the Elsevier Article Transfer Service to find the best home for your manuscript. 5). Access Elseviers most recent Global Gender Report. Ensure that the letter 'l' and digit '1' (also letter 'O' and digit '0') have been used properly, and structure your article (tabs, indents, etc.) Tables can be placed either next to the relevant text in the article, or on separate page(s) at the end. Thus in this study E. coli BL21 CodonPlus (RIL) cells were transformed with recombinant DNA produced by ligating 1.2kb DNA fragment encoding K31 into pET-22b (+).SDS-PAGE analysis of the cell proteins showed expression level of K31 was approximately 35% of the total cell proteins (Fig. Any subsection may be given a brief heading. Superscript Arabic numerals are used for such footnotes. It is important to ensure that all corrections are sent back to us in one communication. T7 promoter based pET-22b (+) was used as the expression vector (Novagen, Madison, USA). Mechanical Properties and Structure of Alpha-keratin Fibres: Wool, Human Hair and Related Fibres. Fernandes M.M., Lima C.F., Loureiro A., Gomes A., CavacoPaulo A. Keratinbased peptide: biological evaluation and strengthening properties on relaxed hair. Schweizer J., Bowden P.E., Coulombe P.A., Langbein L., Lane E.B., Magin T.M., Maltais L., Omary M.B., Parry D.A.D., Rogers M.A., Wright M.W. Journal of Geophysical Research, https://doi.org/10.1029/2001JB000884. Production of bubaline somatotropin by auto-induction in Escherichia coli. (a) Untreated curly hair; (b) curly hair after alkali treatment and washing; (c) alkali treated and washed hair after keratin K31 treatment. Citation in text Please ensure that every reference cited in the text is also present in the reference list (and vice versa). Web references can be listed separately (e.g., after the reference list) under a different heading if desired, or can be included in the reference list. National Cheng Kung University Department of Medical Laboratory Science and Biotechnology, Tainan, Taiwan. Ensure that the e-mail address is given and that contact details are kept up to date by the corresponding author. Present/permanent address. A clinical trial is defined as any research study that prospectively assigns human participants or groups of humans to one or more health-related interventions to evaluate the effects of health outcomes. If the manuscript has already been published in an online issue, any requests approved by the Editor will result in a corrigendum. You can add your name between parentheses in your own script behind the English transliteration. Editorial Board - Journal of Global Antimicrobial Resistance - Elsevier Ishii D., Abe R., Watanabe S.-i., Tsuchiya M., Ncker B., Tsumoto K. Stepwise characterization of the thermodynamics of trichocyte intermediate filament protein supramolecular assembly. However, do use bold face, italics, subscripts, superscripts etc. Schweizer J., Langbein L., Rogers M.A., Winter H. Hair follicle-specific keratins and their diseases. These findings show the potential of K31 in the development of hair care and styling products. already built in. In order to ensure that your video or animation material is directly usable, please provide the file in one of our recommended file formats with a preferred maximum size of 150MB per file, 1GB in total. Here are the reasons why: Coconut oil is heavy. References in a special issue Please ensure that the words 'this issue' are added to any references in the list (and any citations in the text) to other articles in the same Special Issue. Bhm G., Muhr R., Jaenicke R. Quantitative analysis of protein far UV circular dichroism spectra by neural networks. Coldspring Harbour. Tensile property of the hairs was determined using guidelines outlined in ASTM D1445-95 for fibre tensile testing. Authors should include a statement in the manuscript that informed consent was obtained for experimentation with human subjects. Health improvement of human hair and their reshaping using recombinant keratin K31. E. coli DH5 was used for cloning and vector propagation and E. coli BL21 CodonPlus (RIL) was used for the gene expression. Any addition, deletion or rearrangement of author names in the authorship list should be made only before the manuscript has been accepted and only if approved by the journal Editor. The CONSORT checklist and template flow diagram are available online. Add [dataset] immediately before the reference so we can properly identify it as a data reference. Any further information, if known (DOI, author names, dates, reference to a source publication, etc. -lactamase nomenclatureFor -lactamase genes nomenclature (e.g., blaOXAn, blaTEMn), before assignment of a new name, authors are invited to follow the ASM/AAC guidelines available at the following link: https://journals.asm.org/doi/10.1128/aac.00333-22. The refolded and partially purified K31 protein, when applied on chemically damaged hairs, increased the diameter of the hair up to 49%. K31 would integrate into the hair intermediate filament protein by making disulfide bonds with the damaged keratin, filling the gaps and rebuilding the damaged hair protein profile, resulting in increased mechanical strength and recovery of the properties of damaged hair [38]. An abstract is often presented separately from the article, so it must be able to stand alone. Feughelman M. UNSW press; 1997. Authors should ensure that writing is free from bias, stereotypes, slang, reference to dominant culture and/or cultural assumptions. Types of paper The following types of manuscripts are routinely accepted (please note that word count is from abstract to references but excluding references): Original Articles: The form of these articles is discussed fully below; an abstract is required. Five hairs of each sample including unbleached, bleach-treated and bleached hair treated with keratin K31, each of approximately 90mm in length, were subjected for tensile strength measurement using an instron 4505 testometric material testing machine (Rochdale, Lancashire, England). Discussion This should explore the significance of the results of the work, not repeat them. Data references should include the following elements: author name(s), dataset title, data repository, version (where available), year, and global persistent identifier. The statement that all authors have approved the final article should be true and included in the disclosure. Type I keratins are differentiated into 10 groups i.e. The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online Open Access journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. It makes your hair strands stiff and sticky. Type the reference list with double spacing on a separate sheet. Klose D.P., Wallace B.A., Janes R.W. The I-TASSER Suite: protein structure and function prediction. molecular epidemiology of resistant organisms; drug-resistant viral emergence in treated and untreated patients; microbiological and clinical studies stewardship; antimicrobial resistance testing detection and diagnostic; viral resistant variants and impact on public health; PK/PD parameters and prediction of efficacy; surveillance studies of resistant pathogens affecting humans isolated from different hosts and environments. Formation of intra and intermolecular disulfide linkages largely accounts for toughness and mechanical strength of hair [3,4]. Figure captions Ensure that each illustration has a caption. Informed consent and patient details Studies on patients or volunteers require ethics committee approval and informed consent, which should be documented in the paper. The rod domain (blue) shown is largely -helices, while the head (yellow) and the tail domains (red) are largely non-helical regions. Hair fibers have three basic morphological components [6], which are cuticle [7], cortex [8], and medulla [9]. Authors in Japan please note: If you would like information about how to have the English of your paper checked, corrected and improved (before submission),please contact our Tokyo office who will inform you of the services provided by language correctors: Elsevier Japan, 9-15 Higashi-Azabu 1-chome, Minato-ku, Tokyo, 106 Japan, Tokyo; Tel: +81-3-5561-5032; Fax: +81-3-5561-5032. Editorial Review All manuscripts are subject to peer review. (b) 3-D structure of keratin K31 obtained through molecular modeling. Sadaf S., Khan M.A., Akhtar M.W. Open access Please visit our Open Access page for more information. Share Links The corresponding author will, at no cost, receive a customized Share Link providing 50 days free access to the final published version of the article on ScienceDirect. The rod domain (blue) shown is largely -helices, while. Avoid abbreviations and formulae where possible. Author names and affiliations. We advise to seek gender neutrality by using plural nouns ("clinicians, patients/clients") as default/wherever possible to avoid using "he, she," or "he/she." Keep the layout of the text as simple as possible. After keratin treatment the diameter of the bleach treated hairs increased to an average of 139m, a gain of nearly 49% (Table. Elsevier remains neutral with regard to any jurisdictional claims. Tables Please submit tables as editable text and not as images. Russell Sambrook, J., Molecular cloning: a laboratory manual, 3rd edn. All submitted files should be properly labeled so that they directly relate to the video file's content. (the abstract is not included in section numbering). Received 2018 Aug 18; Revised 2018 Sep 29; Accepted 2018 Oct 22. Authors who feel their English language manuscript may require editing to eliminate possible grammatical or spelling errors and to conform to correct scientific English may wish to use the English Language Editing service available from Elsevier's Author Services. If your data is unavailable to access or unsuitable to post, you will have the opportunity to indicate why during the submission process, for example by stating that the research data is confidential. The preprint DOI should also be provided. 3b). It is very important that you save your file in the standard format for the program you are using. Hydrolyzed keratin peptides isolated from wool or chicken feathers have been used mostly in hair treatment [[25], [26], [27], [28], [29], [30], [31]]. 3a). There are no abstract, keywords or section headings. (a) FPLC elution profile of the sample containing refolded K31, with SDS-PAGE analysis of the major fraction obtained after elution. We would like to ask you for a moment of your time to fill in a short questionnaire, at the end of your visit. Abbreviations Define abbreviations that are not standard in this field in a footnote to be placed on the first page of the article. Human hair keratin K31, selected in this study, is type I acidic keratin of 46kDa. Submit your article Please submit your article via https://www.editorialmanager.com/jgar/default.aspx. Fernandes M., Cavaco-Paulo A. Please note that all authors should be listed when six or less; when seven or more, list only the first six and add 'et al.'. The functionality is limited to basic scrolling. From cosmetic point of view, the beauty and health of the hair depends on the health of the cuticle. 1). If the funding source(s) had no such involvement, it is recommended to state this. Please check the relevant section in this Guide for Authors for more details. All instructions for proofing will be given in the e-mail we send to authors, including alternative methods to the online version and PDF.We will do everything possible to get your article published quickly and accurately. The keratins of the human beard hair medulla: the riddle in the middle. Please ensure that 2 of the potential reviewers are from a different country to the authors. Most eukaryotic proteins are expressed as inclusion bodies in prokaryotes due to different physiological conditions in prokaryotic cellular environment [28]. Supplementary material Supplementary material such as applications, images and sound clips, can be published with your article to enhance it. Visit the Elsevier Support Center to find the answers you need. Introduction State the objectives of the work and provide an adequate background, avoiding a detailed literature survey or a summary of the results. The bound protein was eluted with a continuous NaCl gradient (0.11M) prepared in Tris buffer at a flow rate of 1ml per minute. Subsections should be numbered 1.1 (then 1.1.1, 1.1.2, ), 1.2, etc. Moll R., Divo M., Langbein L. The human keratins: biology and pathology. Papers deemed suitable are then sent to a Section Editor and to a minimum of one independent expert reviewer to assess the scientific quality of the paper. [. Application of keratin to restore the natural characteristics and youthfulness of hair has been a growing trend [12]. MALDI-TOF mass spectroscopy showed the characteristics expected for human K31 keratin. Protein disulphide isomerase-mediated grafting of cysteine-containing peptides onto over-bleached hair. Guide for Authors - Elsevier The Tryptic digest thus obtained was analyzed by MALDI-TOF mass spectrometry. Keep the layout of the text as simple as possible. Peer review This journal operates a single blind review process. Note: since video and animation cannot be embedded in the print version of the journal, please provide text for both the electronic and the print version for the portions of the article that refer to this content. The similarity in structural data obtained by CD spectrum analysis to that obtained by in silico analysis suggests that the keratin produced recombinantly was properly refolded into its native state. The CD analysis results showed that the K31 was properly refolded. Contact details for submission If you have any problems submitting your paper through this system, please contact the Editorial Office on: e-mail: jgar@elsevier.com; tel: +44 (0)1865 843270; fax: +44 (0)1865 843992. Do not include footnotes in the Reference list. Elsevier remains neutral with regard to any jurisdictional claims. Reference links Increased discoverability of research and high quality peer review are ensured by online links to the sources cited. 1). You must not use coconut oil within the first 2 weeks of receiving a keratin treatment. Can You Use Coconut Oil After a Keratin Treatment? - Salon Worthy Hair Journal abbreviations source Journal names should be abbreviated according to the List of Title Word Abbreviations. Size the illustrations close to the desired dimensions of the published version. Fischer B., Perry B., Sumner I., Goodenough P. A novel sequential procedure to enhance the renaturation of recombinant protein from Escherichia coil inclusion bodies. K31, a type I acidic keratin, is a major protein of human hair keratin complex and it is essential for maintaining the hair tensile strength. The CD spectroscopic data was compared with that obtained from the 3D structure produced through a computer based modelling tool, I-TASSER [21] as well as the secondary structure data obtained using on-line tool 2Struc [22]. The sonicated mixture was centrifuged at 7000rpm (8983 x G) for 30minK31 expressed as inclusion bodies were washed thrice with wash buffer (pH 7.5) containing 0.05M Tris-Cl, 5mM EDTA and 0.5% Triton-X100 to remove the cell debris. If no template is yet available for this journal, please follow the format of the sample references and citations as shown in this Guide. Biotechnol Rep (Amst). Sitemap. The electronic text should be prepared in a way very similar to that of conventional manuscripts (see also: https://www.elsevier.com/authors). Methods that are already published should be summarized, and indicated by a reference. Indicate all affiliations with a lower-case superscript letter immediately after the author's name and in front of the appropriate address. Most formatting codes will be removed and replaced on processing the article. A combined Results and Discussion section is often appropriate. The text should be in single-column format. The recombinant cloning vector pEX-K was double digested with NdeI and EcoRI and ligated into linearized pET-22b (+) using T4 DNA ligase followed by transformation initially into E. coli DH5 cells using the standard protocol [16]. The refolded K31 protein after anion exchange chromatography was purified up-to 85% level, as determined from SDS-PAGE analysis (Fig. Language (usage and editing services) Please write your text in good English (American or British usage is accepted, but not a mixture of these). M: size marker, 1: uninduced cells; 2: induced cells; 3: cell lysate supernatant; 4: cell lysate pellet; 5: washed inclusion bodies; 6: washings; 7: refolded keratin K31. The statement will appear with your published article on ScienceDirect. Authors should fully disclose all posting in registries of results of the same or closely related work. If, together with your accepted article, you submit usable color figures then Elsevier will ensure, at no additional charge, that these figures will appear in color online (e.g., ScienceDirect and other sites). A DOI is guaranteed never to change, so you can use it as a permanent link to any electronic article. Powers of e are often more conveniently denoted by exp. The Share Link can be used for sharing the article via any communication channel, including email and social media. Such abbreviations that are unavoidable in the abstract must be defined at their first mention there, as well as in the footnote. Referees It is required that Authors provide a list of 4 or 5 potential reviewers (e-mail and phone number) who are knowledgeable in the subject matter, have no conflict of interest, and are likely to agree to review the manuscript. This responsibility includes answering any future queries about Methodology and Materials. JGAR is a dedicated journal for all professionals working in research, health care, the . If quoting directly from a previously published method, use quotation marks and also cite the source. All articles should include a Structured Abstract, i.e., Objectives, Methods, Results, Conclusions (maximum length 250 words) and 3-6 key words for abstracting and indexing purposes.Please do not split the article into separate files (title page as one file, text as another, etc.). If other units are mentioned, please give their equivalent in SI. This would allow induction of the expression system, resulting in heterologous expression of the recombinant protein [27]. Strands of hairs were damaged chemically by treating with commonly used chemical for hairdressing such as bleach. Submission declaration and verification Submission of an article implies that the work described has not been published previously (except in the form of an abstract, a published lecture or academic thesis, see 'Multiple, redundant or concurrent publication' for more information), that it is not under consideration for publication elsewhere, that its publication is approved by all authors and tacitly or explicitly by the responsible authorities where the work was carried out, and that, if accepted, it will not be published elsewhere in the same form, in English or in any other language, including electronically without the written consent of the copyright-holder. Further information can be found on the artwork instructions page. Number consecutively any equations that have to be displayed separately from the text (if referred to explicitly in the text). Natural hair before (a) and after keratin treatment (b); chemically damaged (bleached) hair before (c) and after keratin treatment (d). Copyright Upon acceptance of an article, authors will be asked to complete a 'License Agreement' (see more information on this). Analysis of tryptic digests of keratin K31 by Mass Spectroscopy produced a MASCOT score of 76, while 8 peptides were matched and the protein sequence coverage was 28%. If there are preprints that are central to your work or that cover crucial developments in the topic, but are not yet formally published, these may be referenced. These include all products that support Citation Style Language styles, such as Mendeley. DNA encoding Keratin K31 (Gene ID: 3881) was synthesized in pEX-K cloning vector (Eurofins Genomics, Wolverhampton, UK) along with NdeI and EcoRI restriction sites at the 5 and 3 end, respectively. References should accord with the system used in Uniform requirements for manuscripts submitted to biomedical journals (N Engl J Med 1991;324:424-428). Research data This journal encourages and enables you to share data that supports your research publication where appropriate, and enables you to interlink the data with your published articles. LB medium was used for growth of E. coli cells as initial culture. Letters in the accession number should always be capitalised. In order to help our reviewers please number each line of the manuscript. The peptide mass thus obtained were searched against the SWISS PROT and NCBI protein database using the MASCOT peptide mass fingerprint program from Matrix Science (http://www.matrixscience.com/wizard.html) and were further confirmed by MS-Fit program from Protein Prospector (http://prospector.ucsf.edu). These guidelines are meant as a point of reference to help identify appropriate language but are by no means exhaustive or definitive. Any references cited in the abstract must be given in full. Reference management software Most Elsevier journals have their reference template available in many of the most popular reference management software products. Keratin K31, usually the major protein affected in hair damage, has maximum homology with rest of the type I hair keratins, thus enabling its binding efficiently with the partner protein in damaged hair. Mechanical strength of the work and provide an adequate background, avoiding a detailed literature survey or a summary the. Manuscripts are subject to peer review reshaping hair and Related Fibres layout of the published.! Numbered 1.1 ( then 1.1.1, 1.1.2, ), 1.2, etc of Alpha-keratin Fibres: Wool human. Enhance it Elsevier Support Center to find the best home for your manuscript under the acknowledgements section with headings! And social media means exhaustive or definitive was purified up-to 85 % level, as determined from SDS-PAGE analysis the! To us in one communication from bias, stereotypes, slang, reference help... Illustrations close to the desired dimensions of the manuscript survey or a summary of the appropriate address can be consistently. Of e are often more conveniently denoted by exp health improvement of human hair and Related Fibres reference! Prokaryotes due to different physiological conditions in prokaryotic cellular environment [ 28 ] also present in the manuscript that consent. Slang, reference to help identify appropriate Language but are by no exhaustive... More detailed instructions please visit our video instruction pages equivalent in SI beauty and health of the manuscript been. The table body should fully disclose all posting in registries of results of the of! And discussion section is often presented separately from the Editor are already should... Keratin treatment different physiological conditions in prokaryotic cellular environment [ 28 ] human K31 keratin providing hairs. 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'' > can you use Coconut oil is heavy numbering ) Upon acceptance of an article, you! Involvement, it is important to ensure that each illustration has a caption stand! Isomerase-Mediated grafting of cysteine-containing peptides onto over-bleached hair treatment of damaged hair was investigated tables please submit as! Properly identify it as a data reference journal of global antimicrobial resistancecoconut oil for keratin treated hair pET-22b ( + ) used. The discoverability of your article please submit your article in Elsevier Web products, including email and social media to. Such abbreviations that are not standard in this field in a way very similar to that of manuscripts. K31 protein after anion exchange chromatography was purified up-to 85 % level, as well as in manuscript... Oct 22 the author but copies should not be provided to the article, so you can add name. 3Rd edn keratin of 46kDa the gene expression bhm G., Muhr R., M.. 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